玉米大斑病菌和小斑病菌交配型多重PCR 检测方法的建立与应用

代玉立,甘林,滕振勇,杨静民,祁月月,石妞妞,陈福如,杨秀娟

Establishment and Application of a Multiple PCR Method to Detect Mating Types of Exserohilum turcicum and Bipolaris maydis

YuLi DAI,Lin GAN,ZhenYong TENG,JingMin YANG,YueYue QI,NiuNiu SHI,FuRu CHEN,XiuJuan YANG

表2 玉米大斑病菌和小斑病菌交配型多重PCR检测的特异引物

Table 2 Specific primers for multiple PCR to detect mating types of E. turcicum and B. maydis

引物名称 Primer name 引物序列 Primer sequence (5′→3′) 退火温度 Tm (℃) 产物大小 Size (bp) 目标基因 Target gene StMAT01-2 F: TGCCTTTGTTGGATTTCG 57.2 816 E. turcicum MAT1-1 R: CATCGTTCTGGCTGTGGG StMAT02-3 F: TACACCAAACAACATCGCTCCT 57.2 132 E. turcicum MAT1-2 R: TCGGCGTCGTAGAACAAG ChMAT01-3 F: ACCACGAGACAACATACGC 55.0 490 B. maydis MAT1-1 R: GTTTGAGGTGAGGGCAGA ChMAT02-2 F: GGCGAAGTTTGTGGGTTT 55.0 136 B. maydis MAT1-2 R: GCGATGTCTGGGCTGATT