玉米质膜内在蛋白ZmPIP2;6响应渗透、盐和 干旱胁迫的功能鉴定
周练,熊雨涵,洪祥德,周京,刘朝显,王久光,王国强,蔡一林(
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Functional Characterization of a Maize Plasma Membrane Intrinsic Protein ZmPIP2;6 Responses to Osmotic, Salt and Drought Stress
ZHOU Lian,XIONG YuHan,HONG XiangDe,ZHOU Jing,LIU ChaoXian,WANG JiuGuang,WANG GuoQiang,CAI YiLin()
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图4. ZmPIP2;6超表达转基因株系的检测
A：ZmPIP2;6超表达载体的T-DNA序列示意图;B：ZmPIP2;6超表达T1代转基因株系的PCR检测;1—5：独立的转基因株系,MK：DNA Marker,PC：质粒正对照,NC：野生型负对照;C：3个独立的ZmPIP2;6超表达T2代转基因株系的qRT-PCR分析
Fig. 4. Generation of ZmPIP2;6 overexpression transgenic lines
A: Schematic illustration of T-DNA sequence of ZmPIP2;6 overexpression vector; B: PCR detection of T1 generation of ZmPIP2;6 overexpression transgenic lines; 1-5: Five independent transgenic lines; MK: DNA Marker, PC: Positive control, NC: WT negative control; C: qRT-PCR analysis of three representative ZmPIP2;6 overexpression transgenic lines
