重组鸭瘟病毒载体中筛选高效表达鸭坦布苏病毒E蛋白启动子
陈柳(
),倪征,余斌,华炯钢,叶伟成,云涛,刘可姝,朱寅初,张存()
),倪征,余斌,华炯钢,叶伟成,云涛,刘可姝,朱寅初,张存()
Optimized Promoter Regulating of Duck Tembusu Virus E Protein Expression Delivered by a Vectored Duck Enteritis Virus in vitro
CHEN Liu(),NI Zheng,YU Bin,HUA JiongGang,YE WeiCheng,YUN Tao,LIU KeShu,ZHU YinChu,ZHANG Cun()
),NI Zheng,YU Bin,HUA JiongGang,YE WeiCheng,YUN Tao,LIU KeShu,ZHU YinChu,ZHANG Cun()
图5. Western blotting方法检测重组病毒感染细胞（A）和浓缩上清（B）中Es的表达
Fig. 5. Western blotting analysis of Es protein expressed in recombinant virus-infected CEFs
1: rDEV-Es; 2: rDEV-pSV40-Es; 3: rDEV-pCAG-Es; 4: rDEV-pRSV-Es; 5: rDEV-p1.8(MDV) -Es; 6: rDEV-pgB(MDV)-Es; M: EasySee Western Marker (90, 75, 60,40, 25 kD); 7: rDEV-EF1
