重组鸭瘟病毒载体中筛选高效表达鸭坦布苏病毒E蛋白启动子
陈柳(
),倪征,余斌,华炯钢,叶伟成,云涛,刘可姝,朱寅初,张存()
),倪征,余斌,华炯钢,叶伟成,云涛,刘可姝,朱寅初,张存()
Optimized Promoter Regulating of Duck Tembusu Virus E Protein Expression Delivered by a Vectored Duck Enteritis Virus in vitro
CHEN Liu(),NI Zheng,YU Bin,HUA JiongGang,YE WeiCheng,YUN Tao,LIU KeShu,ZHU YinChu,ZHANG Cun()
),NI Zheng,YU Bin,HUA JiongGang,YE WeiCheng,YUN Tao,LIU KeShu,ZHU YinChu,ZHANG Cun()
图2. 重组BAC突变体克隆的PCR鉴定
泳道1—6 是以DEV-tk-F和DEV-tk-R引物对扩增片段;7—12 是以JD-F和JD-R引物对扩增片段。M1：DL2000(2000,1000, 750, 500, 250, 100 bp); 1. pDEV-EF1 (553 bp); 2. pDEV-pSV40. Es (553 bp); 3. pDEV- pRSV. Es (553 bp); 4. pDEV-pCAG. Es(553 bp); 5. pDEV-p1.8k (MDV). Es (553 bp); 6. pDEV-pgB(MDV). Es (553 bp); M2: 250 bp marker(4500, 3000, 2250, 1500, 1000, 750, 500, 250 bp); 7. pDEV-EF1(641 bp); 8. pDEV-pSV40. Es(2747 bp); 9. pDEV-pRSV. Es(2745 bp); 10. pDEV- pCAG. Es(3989 bp); 11. pDEV-p1.8k(MDV). Es (2687 bp);12. pDEV-pgB (MDV). Es (3037 bp)
Fig. 2. Analysis of recombinant BAC mutants by PCR amplification
