重组鸭瘟病毒载体中筛选高效表达鸭坦布苏病毒E蛋白启动子

陈柳,倪征,余斌,华炯钢,叶伟成,云涛,刘可姝,朱寅初,张存

Optimized Promoter Regulating of Duck Tembusu Virus E Protein Expression Delivered by a Vectored Duck Enteritis Virus in vitro

CHEN Liu,NI Zheng,YU Bin,HUA JiongGang,YE WeiCheng,YUN Tao,LIU KeShu,ZHU YinChu,ZHANG Cun

表1 本文所用引物

Table 1 Primers used in this study

引物名称 Primer 序列 Sequence 引入位点 Sequence introduced pCAG(MluI+) 5′-cgACGCGTTAGTTATTAATAGTAATCAATTACG-3′ Mlu I PCAG(NheI-) 5′-ctaGCTAGCGCCGCCGGTCACACGCCAGAAGCC-3′ Nhe I pRSV(BglII+) 5′-gaAGATCTCTGCTCCCTGCTTGTGTGTTG-3′ Bgl II pRSV(NheI-) 5′-ctaGCTAGCGTGCACACCAATGTGGTGAATG-3′ Nhe I pSV40(MluI+) 5′-cgACGCGTCTGTGGAATGTGTGTCAGTTAGG-3′ Mlu I pSV40(NheI-) 5′-ctaGCTAGCCGAAAATGGATATACAAGCTCCCGG-3′ Nhe I F(MDV p1.8k BglII+) 5′-gaAGATCTTCGAGGCCACAAGAAATTAC-3′ Bgl II R(MDV p1.8k NheI-) 5′-ctaGCTAGCGAGCATCGCGAAGAGAGAAG-3′ Nhe I F(MDV gB BglII+) 5'-cgAGATCTCAAGTCTCACTCACAAATTTTTTC-3′ Bgl II R(MDV gB NheI-) 5′-ctaGCTAGCAGTGAGATGATCTTAATGATGC-3′ Nhe I pDEV vac-in-s(p1.8k,pgB) 5′-TACTAATTTAAGTGTGCAGCCTGGTTAACTGTATTATGCGCGGAGTGACGTCGACGGATCGGG-3′ pDEV vac-in-s 5′-TACTAATTTAAGTGTGCAGCCTGGTTAACTGTATTATGCGCGGAGCGATGTACGGGCCAGATA-3′ pDEV vac-in-as 5′- TCCGTAGTCTGGCCGGCAGTATGTTGGTGTTTAGTACTCCAAACCCA TAGAGCCCACCGCATCCCC-3′ JD-F 5′-CTACCACAAGCGTCATCAACCA-3′ JD-R 5′-TGTCCATTACCAAATCCGAAAA-3′ DEV-tk-F 5′- GCTTCCCAGCAGCTCGTT-3′ DEV-tk-R 5′- TCTCGTACTTCAGCGGCACA-3′ Dev UL44(BamHI+) 5′-cgGGATCCATGGGGCCATTAGTGATGGTTG-3′ BamH I Dev UL44 (XhoI-) 5′-ccgCTCGAGTCAAATAATATTGTCTGCTTTATC-3′ XhoI