藤茶查尔酮合成酶基因AgCHS1的克隆及功能鉴定
许明(
),林世强,倪冬昕,伊恒杰,刘江洪,杨志坚,郑金贵()
),林世强,倪冬昕,伊恒杰,刘江洪,杨志坚,郑金贵()
Cloning and Function Characterization of Chalcone Synthase Gene AgCHS1 in Ampelopsis grossedentata
XU Ming(),LIN ShiQiang,NI DongXin,YI HenJie,LIU JiangHong,YANG ZhiJian,ZHENG JinGui()
),LIN ShiQiang,NI DongXin,YI HenJie,LIU JiangHong,YANG ZhiJian,ZHENG JinGui()
图7. 过量表达AgCHS1对烟草花色、花青素和黄酮醇含量的影响
A：转基因烟草的PCR鉴定;B：花瓣表型;C：花瓣AgCHS1表达量;D：花瓣相对花青素含量;E：花瓣的相对黄酮醇含量
M：DL1000 DNA标准分子量;P：质粒对照,WT：野生型;OE1—OE5：转基因株系。不同字母表示在P<0.05水平差异显著
Fig. 7. Efects of overexpression AgCHS1 on the color, anthocyanin and flavonol content of tobacco
A: PCR identification of transgenic tobacco; B: Petal phenotypes; C: AgCHS1 expression in petal; D: Relative anthocyanin content; E: Relative flavonol content;
M: DNA marker DL1000; P: Plasmid control; WT: Wild type; OE1-5: different transgenic lines. Different letters indicate statistical difference (P<0.05)
