藤茶查尔酮合成酶基因AgCHS1的克隆及功能鉴定
许明(
),林世强,倪冬昕,伊恒杰,刘江洪,杨志坚,郑金贵()
),林世强,倪冬昕,伊恒杰,刘江洪,杨志坚,郑金贵()
Cloning and Function Characterization of Chalcone Synthase Gene AgCHS1 in Ampelopsis grossedentata
XU Ming(),LIN ShiQiang,NI DongXin,YI HenJie,LIU JiangHong,YANG ZhiJian,ZHENG JinGui()
),LIN ShiQiang,NI DongXin,YI HenJie,LIU JiangHong,YANG ZhiJian,ZHENG JinGui()
图6. 重组蛋白AgCHS1体外酶促产物的HPLC-MS鉴定
A：重组蛋白AgCHS1酶促产物的色谱图;B：柚皮素标准品色谱图;C：空载体对照;D—E：柚皮素标准品的质谱图;F—G：重组蛋白AgCHS1酶促产物的质谱图
Fig. 6. HPLC-MS identification of in vitro enzymatic reaction product of recombinant protein AgCHS1
HPLC chromatograms of the products in the reactions of AgCHS1 recombinant protein (A); naringen instandard (B) and the empty control (C); D-E: The masss pectrometric spectrum of reaction products; F-G: The mass spectrometric spectrum of the naringenin standard
