藤茶查尔酮合成酶基因AgCHS1的克隆及功能鉴定
许明(
),林世强,倪冬昕,伊恒杰,刘江洪,杨志坚,郑金贵()
),林世强,倪冬昕,伊恒杰,刘江洪,杨志坚,郑金贵()
Cloning and Function Characterization of Chalcone Synthase Gene AgCHS1 in Ampelopsis grossedentata
XU Ming(),LIN ShiQiang,NI DongXin,YI HenJie,LIU JiangHong,YANG ZhiJian,ZHENG JinGui()
),LIN ShiQiang,NI DongXin,YI HenJie,LIU JiangHong,YANG ZhiJian,ZHENG JinGui()
图5. 大肠杆菌表达重组AgCHS1蛋白的Ni柱纯化
M：蛋白Marker;1：离心后的裂解液上清;2：裂解液上清经过Ni柱的流穿;3：Binding buffer洗脱;4：Binding buffer + 60 mmol?L-1咪唑洗脱;5：Binding buffer + 90 mmol?L-1咪唑洗脱;5：Binding buffer + 250 mmol?L-1咪唑洗脱
Fig. 5. Ni column purification of recombinant AgCHS1 protein expressed in E. coli
M: Protein Marker; 1: Centrifuged supernatant; 2: Flow through; 3: binding buffer elution; 4: Binding buffer + 60 mmol?L-1 imidazole elution; 5: Binding buffer + 90 mmol?L-1 imidazole elution; 5: Binding buffer + 250 mmol?L-1 imidazole elution
