藤茶查尔酮合成酶基因AgCHS1的克隆及功能鉴定
许明(
),林世强,倪冬昕,伊恒杰,刘江洪,杨志坚,郑金贵()
),林世强,倪冬昕,伊恒杰,刘江洪,杨志坚,郑金贵()
Cloning and Function Characterization of Chalcone Synthase Gene AgCHS1 in Ampelopsis grossedentata
XU Ming(),LIN ShiQiang,NI DongXin,YI HenJie,LIU JiangHong,YANG ZhiJian,ZHENG JinGui()
),LIN ShiQiang,NI DongXin,YI HenJie,LIU JiangHong,YANG ZhiJian,ZHENG JinGui()
图2. AgCHS1与其他物种CHS氨基酸序列比对分析
AgCHS1：藤茶Ampelopsis grossedentata;VvCHS：葡萄 Vitis vinifera NP_001267879.1;GhCHS1：陆地棉 Gossypium hirsutum CV72638;OsCHS：水稻 Oryza sativa CAA61955.1;AtCHS：拟南芥Arabidopsis thaliana AAA32771.1;NtCHS：烟草Nicotiana tabacum ANA78328.1。▲：Cys-His-Asn三联活性中心;★：表示苯丙氨酸残基Phe215和Phe265;方框为CHS高度保守特征序列,下划线为丙二酰CoA结合位点 ▲: Cys-His-Asn triple active center; ★: phenylalanine residues Phe215 and Phe265; the highly conserved CHS signature sequence are boxed with black line; the malonyl-CoA binding site are underlined
Fig. 2. Amino acid sequence alignment of AgCHS1 with other CHS enzymes
