藤茶查尔酮合成酶基因AgCHS1的克隆及功能鉴定

许明,林世强,倪冬昕,伊恒杰,刘江洪,杨志坚,郑金贵

Cloning and Function Characterization of Chalcone Synthase Gene AgCHS1 in Ampelopsis grossedentata

XU Ming,LIN ShiQiang,NI DongXin,YI HenJie,LIU JiangHong,YANG ZhiJian,ZHENG JinGui

表1 本试验中所用的引物序列

Table 1 List of primers used in this study

引物名称 Primer name 序列（5'-3'） Primer sequence (5'-3') 用途 Purpose AgCHS1-F GGATCCATGGTGTCGGTGCAGGAAATCAGA ORF序列扩增、载体构建 AgCHS1-R GAGCTCTCAGTGAGCCAGTGGTGCAGAC Amplification of ORF sequence and vector construction AgCHS1-P1 ACTCCAGCCAACTGTGTCCA 转基因烟草的PCR鉴定 AgCHS1-P2 TGAGACCCACTTCACGCAA PCR identification of transgenic tobacco AgCHS1-qF GCCTCAAACCCTCCGTCA 实时荧光定量PCR Quantitative real-time PCR AgCHS1-qR GACCCACGAGTGAATCCAAGT Quantitative real-time PCR NtEF1α-qF AATTTTGACCAAGATCGACAGG 烟草内参基因 Control NtEF1α-qR CAGCAACAGTTTGACGCATG Reference gene in tobacco AgGAPDH-qF CATCTCAGCCCCAAGCAA 藤茶内参基因 AgGAPDH-qR GTGGCAGTAATGGAGTGAACAG Reference gene in A.grossedentata