玉米大斑病菌<i>Septin</i>基因家族的鉴定与表达模式分析

玉米大斑病菌Septin基因家族的鉴定与表达模式分析

龙凤,王擎,朱行,王建霞,申珅,刘宁,郝志敏,董金皋

Identification and Expression Pattern Analysis of Septin Gene Family of Setosphaeria turcica

LONG Feng,WANG Qing,ZHU Hang,WANG JianXia,SHEN Shen,LIU Ning,HAO ZhiMin,DONG JinGao

表2 RT-qPCR反应体系

Table 2 RT-qPCR reaction system

反应组分 Reaction component	反应体系 Reaction system	终浓度 Final concentration
2×Fast Super EvaGreen Master Mix	10 μL	1×
F, R primers	1 μL	0.1-0.5 μmol·L^-1 each
cDNA (500 ng·μL^-1) template	1 μL
H₂O	补足至20 μL Up to 20 μL