转phyA2、ZmTMT和Bar玉米的获得及其特性分析
姚兴兰(
),杨文竹,罗彦忠,陈茹梅,王磊(),张兰()
),杨文竹,罗彦忠,陈茹梅,王磊(),张兰()
Acquisition and Characteristic Analysis of Transgenic Maize with phyA2, ZmTMT, and Bar
YAO XingLan(),YANG WenZhu,LUO YanZhong,CHEN RuMei,WANG Lei(),ZHANG Lan()
),YANG WenZhu,LUO YanZhong,CHEN RuMei,WANG Lei(),ZHANG Lan()
图3. 成熟籽粒中phyA2、ZmTMT和Bar蛋白的Western blot分析
A：ZmTMT蛋白的表达分析;B：Bar蛋白的表达分析;C：phyA2蛋白的表达分析。WT：野生型郑58;TPB1、TPB2：转基因纯合株系;Marker：蛋白分子量标准;ZmTMT CK：原核表达的融合ZmTMT蛋白阳性对照;Bar CK：Bar蛋白阳性对照;phyA2 CK：已报道的转基因玉米表达的phyA2蛋白阳性对照[
Fig. 3. Western blot analysis of phyA2, ZmTMT and Bar in mature maize seeds
A, B, and C show the protein accumulation of ZmTMT, Bar and phyA2, respectively; WT: Wild type Zheng58; TPB1, TPB2: Transgenic homozygous lines; ZmTMT CK: Positive control of ZmTMT; Bar CK: Positive control of Bar; phyA2 CK: Positive control of phyA2
