以本地早橘和槾橘为母本倍性杂交创制柑橘三倍体
解凯东1(),彭珺1,袁东亚1,强瑞瑞1,谢善鹏1,周锐1,夏强明1,伍小萌1,柯甫志2,刘高平3,GROSSER Jude W4,郭文武1()
Production of Citrus Triploids Based on Interploidy Crossing with Bendizao and Man Tangerines as Female Parents
XIE KaiDong1(),PENG Jun1,YUAN DongYa1,QIANG RuiRui1,XIE ShanPeng1,ZHOU Rui1,XIA QiangMing1,WU XiaoMeng1,KE FuZhi2,LIU GaoPing3,GROSSER Jude W4,GUO WenWu1()

图1. 槾橘×NS组合幼胚离体挽救培养、植株再生及田间嫁接
a:授粉后85 d的种子(下)及对照种子(上);b:接种于萌发培养基的种子;c:种子萌发形成胚状体;d:生芽培养;e:生根培养;f:植株炼苗;g:移入小塑料杯的三倍体;h:移入温室的三倍体群体;i:嫁接至田间的三倍体植株(枳砧)

Fig. 1. Embryo rescue, plant regeneration and grafting of triploid plants from the cross of Man tangerine × NS
a: Seeds of ‘Man tangerine × NS’ obtained at 85 DAP (lower) and open-pollinated seeds (upper); b: Seeds cultured in medium for germination; c: Germinated embryos on the medium; d: Embryonoids transferred on the medium for shoot induction; e: A shoot transferred to the medium for root induction; f: Plantlet acclimation; g: A triploid plantlet in plastic pot; h: Triploid population in greenhouse; i: Grafted triploids in the field