干扰KISS1基因对猪卵巢颗粒细胞功能的影响
陆思羽,何颖婷,周小枫,辛晓萍,张爱玲,袁晓龙,张哲,李加琪

Effect of KISS1 Interference on the Function of Porcine Granulosa Cells in Porcine Ovary
LU SiYu,HE YingTing,ZHOU XiaoFeng,XIN XiaoPing,ZHANG AiLing,YUAN XiaoLong,ZHANG Zhe,LI JiaQi
表1 定量引物
Table 1 Quantitative primer
引物名称 Primer name Gene ID 引物序列 Primer sequence 产物大小 Size (bp)
qRT-KISS1 100145896 F: AACCAGCATCTTCTCACCAGG 192
R: CTTTCTCTCCGCACAACGC
qRT-PIK3CG 396979 F: AACGGGCTTTGAGATAGTGAA 184
R: AAGTTGCTTGGTTGGTGGATA
qRT-PIK3C1 5290 F: CAAGTGAGAATGGTCCGAATG 152
R: GTGGAAGAGTTTGCCTGTTTT
qRT-PDK1 100286871 F: AAATCACCAGGACAGCCAATA 190
R: CTTCTCGGTCACTCATCTTCAC
qRT-AKT1 100126861 F: CTGCACAAACGAGGCGAGT 89
R: CGCTCCTTGTAGCCGATGAA
qRT-FOXO3 733621 F: ACAAACGGCTCACTCTGTCCCA 85
R: GAACTGTTGCTGTCGCCCTTATC
qRT-TSC2 100505408 F: CGAGGTGGTGTCCTACGAGAT 115
R: GAGCAGGCGTTCAATGATGTT
qRT-BAD 100521065 F: AGTCGCCACTGCTCTTACCC 172
R: TCTTGAAGGAACCCTGGAAATC
qRT-ESR1 10013276 F: GATGCCTTGGTCTGGGTGAT 124
R: AGTGTTCCGTGCCCTTGTTA
qRT-ESR2 396697 F: AAGGGAAAAGGAGGATGGGACA 202
R: CAGATAGGGACTGCGTGGAGGT
qRT-Star 396597 F: GGAAAAGACACAGTCATCACCCAT 121
R: CAGCAAGCACACACACGGAAC
qRT-CYP17 100157047 F: AAGCCAAGACGAACGCAGAAAG 228
R: TAGATGGGGCACGATTGAAACC
qRT-3BHSD 445539 F: GGGGCTTCTGTCTTGATTCCA 284
R: GGTTTTCAGTGCTTCCTTGTGC
qRT-17BHSD 397574 F: CCCAACGCAGGAGACTCAAAAT 149
R: CCAGAGCCCATAACGAAGACAGA
qRT-CYP19A 403332 F: GCTGGACACCTCTAACAACCTCTT 91
R: TTGCCATTCATCAAAATAACCCT