两个褐飞虱海藻糖转运蛋白基因的结构及调控海藻糖代谢功能

於卫东,潘碧莹,邱玲玉,黄镇,周泰,叶林,唐斌,王世贵

The Structure Characteristics and Biological Functions on Regulating Trehalose Metabolism of Two NlTret1s in Nilaparvata lugens

YU WeiDong,PAN BiYing,QIU LingYu,HUANG Zhen,ZHOU Tai,YE Lin,TANG Bin,WANG ShiGui

表1 实时荧光定量PCR和dsNlTret1、dsGFP的引物序列

Table 1 Primer sequence used for qRT-PCR and dsNlTret1s, dsGFP

引物名称 Primer name 正向引物 Forward primer (5′-3′) 反向引物 Reverse primer (5′-3′) 产物长度 Length (bp) dsNlTret1-like X1 GCAAACAACAGCGAGCAA CCAAGAGGCACCCATCC 472 dsNlTret1-like X1-T7 T7-GCAAACAACAGCGAGCAA T7-CCAAGAGGCACCCATCC 552 dsNlTret1-2 X1 CTTCGTTCACCAGCACCT CAAATGGCACTTATTATCGTC 547 dsNlTret1-2 X1-T7 T7-CTTCGTTCACCAGCACCT T7-CAAATGGCACTTATTATCGTC 597 dsGFP AAGGGCGAGGAGCTGTTCACCG CAGCAGGACCATGTGATCGCGC 657 dsGFP-T7 T7-AAGGGCGAGGAGCTGTTCACCG T7-CAGCAGGACCATGTGATCGCGC 707 qNl18S CGCTACTACCGATTGAA GGAAACCTTGTTACGACTT 165 qNlTret1-like X1 GTGGGAATCGTGAACATGGG ATGGTCATGAGTGTGCTGGA 101 qNlTret1-2 X1 TATGTGTCGGCTGGGTTCTT CTCTGAGCCAGCACAATGAC 190 qNlTPS1 AAGACTGAGGCGAATGGT AAGGTGGAAATGGAATGTG 154 qNlTPS2 AGAGTGGACCGCAACAACA TCAACGCCGAGAATGACTT 161 qNlTRE1-1 GCCATTGTGGACAGGGTG CGGTATGAACGAATAGAGCC 132 qNlTRE1-2 GATCGCACGGATGTTTA AATGGCGTTCAAGTCAA 178 qNlTRE2 TCACGGTTGTCCAAGTCT TGTTTCGTTTCGGCTGT 197