玉米穗腐病致病禾谷镰孢复合种的遗传多样性、致病力与毒素化学型分析

王宝宝,郭成,孙素丽,夏玉生,朱振东,段灿星

The Genetic Diversity, Pathogenicity, and Toxigenic Chemotypes of Fusarium graminearum Species Complex Causing Maize Ear Rot

WANG BaoBao,GUO Cheng,SUN SuLi,XIA YuSheng,ZHU ZhenDong,DUAN CanXing

表1 本试验所用引物

Table 1 Primers used in this study

引物 Primer 序列 Sequence (5′ to 3′) 退火温度 Tm (℃) 扩增片段大小 Product size (bp) 参考文献 Reference SSR-L1 R: AGAAGAAGGGCAAATGG 57 378 [19] F: TGTCCGAGTCGTCTGAAT SSR-L2 R: GGTGGTGGTTTCCGTGAG 60 214 [19] F: GCGTTGAGATACCCTTTCG SSR-L3 R: CTGCTGCTATCACTTCCAC 58 344 [19] F: TTCACAACGACCGTATTTC SSR-L4 R: GTTGTTGGGTTGCTTGTG 59 160 [19] F: GTCTCGTCCAGATTCCTATT SSR-L5 R: GGTGGTGGTTTCCGTGAG 60 214 [19] F: GCGTTGAGATACCCTTTCG SSR-L6 R: AATAGTGGCGGTGCGTAG 60 386 [19] F: GTCTCCCGAGTAGTTGGC SSR-L7 R: TAGTGGCGGTGCGTAGTG 62 379 [19] F: GTCTCCCGAGTAGTTGGC SSR-L8 R: GGTCCGCAACATAGAGGG 60 403 [19] F: CCGAAGAGGAGAACGAGC SSR-G11 R: TTTTGGGTGTTGAAGAAGCC 56 200-300 [20] F: TTTTTGTTTGCGTCGTTCTG SSR-G12 R: AATGCAGTCGATGGGAAACT 56 200-300 [20] F: GGGTTCCTTGTAAGTGGCTG SSR-G13 R: AGTATCAGACAGGTTGGCCG 56 200-300 [20] F: GTATGCAAAGCAGCGTCGTA SSR-G14 R: GCCCATTACGTTGAGCAAAT 56 200-300 [20] F: TTTTTCGGTCTGGCTATTGG SSR-G15 R: CTCTAGTGGCAGACCCTTCG 56 200-300 [20] F: CTTGACATGTCGCGCTTTTA SSR-G16 R: CTGATCTGCGGACATCTTCA 56 200-300 [20] F: GTTTTGGTTTGCCTGTCGTT SSR-G17 R: TCTGTCGTTGACAAGCAAGC 56 200-300 [20] F: ACGGACCGACGACATAACTC SSR-G18 R: GCGTCGACTAAAGGAACCAG 56 200-300 [20] F: TCCCGACGTTAGAGTGGAGT SSR-G19 R: CCTTGTTCTTTCCACCCTGA 56 200-300 [20] F: ACGAGGACGAACTTGTTGCT 引物 Primer 序列 Sequence (5′ to 3′) 退火温度 Tm (℃) 扩增片段大小 Product size (bp) 参考文献 Reference SSR-G20 R: AAGCTTGGGGGCTAACATCT 56 200-300 F: CAGCTTTGGCGGACATTATT [20] SSR-G21 R: TCACATATTCAACCGACCCA 56 200-300 [20] F: GCTCCGTGTCCTTTCATTTC SSR-G22 R: GACGGATCGTCGGATAGGTA 56 200-300 [20] F: CACTAAGCTGCTCCTCCACC VNTR-F1 F: ACAGGCATCCAAGGACATTT 56 286 [21] R: GTTTGATGGCGCATTCAAAG VNTR-F2 F: GCAGGACCTGGATGATGAA 56 234 [21] R: ATGTGTGCAGCCATGAGATT VNTR-F3 F: ATCTCCCAAGCTGGCTAATT 56 234 [21] R: AGAACCGGCAAAGTTCGATT VNTR-F4 F: TCCGAAGGTAGAAGCGTTGT 56 298 R: TCAAGCCCATCTATGCTGTT [21] VNTR-F5 F: GAGATGGCAACATTATTTGCA 56 252 R: ATTGGCAGCAGGGCTTGATT [21] VNTR-F6 F: AAGAGGGCGTGTCTCTGTTTT 56 215 R: CGCTTCCTTCCTTTCAATTC [21] VNTR-F7 F: AAGACTGGTCAGCAGTAGGGA 56 248 R: TGAGAGCGAGACTGAGCATGA [21] VNTR-F8 F: AAACGTAAACGGATCAACGG 56 210 R: AGATTCGCAACTTTGTGCTG [21] VNTR-F9 F: TGGATATGGTTCCCCAGCT 56 239 R: TACTGACCTTGAGGAGCACCATAC [21] VNTR-F10 F: TATGATGCAGCGAATGCAAC 56 221 [21] R: TAGAGACCTGGCCCATACCA TEF-1α R: ATGGGTAAGGAR-GACAAGAC 58 680 [22] F: GGAR-GTACCAGTSATCATGTT β-tubulin R: GGTAACCAAATCGGTGCTGCTTTC 55 380 [23] F: ACCCTCAGTGTAGTGACCCTTGGC RPB2 R: CCCATRGCTTGYTTRCCCAT 55 900 [24] F: GGGGWGAYCAGAAGAAGGC Tri13a/b R: CTCSACCGCATCGAAGASTCTC 58 583, 644, 859 [25] F: GAASGTCGCARGACCTTGTTTC Tri13F/R R: GGTGTCCCAGGATCTGCG 57 415 [26] F: TACGTGAAACATTGTTGGC Tri303F/R R: GCCGGACTGCCCTATTG 52 586 [27] F: GATGGCCGCAAGTGGA Tri315F/R R: GTCTATGCTCTCAACGGACAAC 58 864 [27] F: CTCGCTGAAGTTGGACGTAA