苦荞ARF基因家族的鉴定及生长素诱导下的表达模式

郝彦蓉,杜伟,侯思宇,王东航,冯红梅,韩渊怀,周美亮,张凯旋,刘龙龙,王俊珍,李红英,孙朝霞

Identification of ARF Gene Family and Expression Pattern Induced by Auxin in Fagopyrum tataricum

HAO YanRong,DU Wei,HOU SiYu,WANG DongHang,FENG HongMei,HAN YuanHuai,ZHOU MeiLiang,ZHANG KaiXuan,LIU LongLong,WANG JunZhen,LI HongYing,SUN ZhaoXia

表1 引物序列

Table 1 Primers of sequences

基因名称 Gene name 正向引物 Forward primers (5′-3′) 反向引物 Reverse primers (5′-3′) FtARF1 AACCCGAGGACACACCTTTC GTTGCCTTGAATAAGCGGGC FtARF2 GCTTGTCGGAGATGACCCTT CTGCACTCCTTCCTTGCTCA FtARF4 CGGGCTGATGTTACCCATGA CCTGCACTCGGCAGTTCATA FtARF14 GCAAATGGGCTTCAACCGAG CCAAGCCATCCATACCAGCA FtARF16 CCAACCTTTGTCTCCGCAAG CGAGGAACAGAAAAACCCCC FtARF17 CGAGGATTGCTTCCCTCCTT CTCCATCCTGTCGTGAGCAA FtARF18 ATAGCATGCACATCGGCCTT ACTTTGCCAGAGGAACGACA FtARF22 CCACGATGTGGTAAACGGGA TTGAGTCCGCACAGAACCTC FtARF23 ACTGAATGGAAGTTCCGCCA AACCGCATCCCCTGAAACAA FtHis ATTCCAGAGGCTTGTTCGTG CATAATGGTGACACGCTTGG