),李健2,王峥2,陈卓1,何航2,邓兴旺2,马力耕1()
),LI Jian2,WANG Zheng2,CHEN Zhuo1,HE Hang2,DENG XingWang2,MA LiGeng1()
图5. Ms1和ThMs1蛋白体外结合磷脂类分子
A、B：MBP-His、MBP-Ms1-His、MBP-ThMs1-His蛋白的纯化和检测;MBP-His、MBP-Ms1-His、MBP-ThMs1-His蛋白覆膜：脂膜P-6001(C)和脂膜P-6002(D)。CL：心磷脂;CS：胆固醇;DAG：二酰甘油;LPA：溶血磷脂酸;LPC：溶血磷脂酰胆碱;PE：磷脂酰乙醇胺;PG：磷脂酰甘油;PI：磷脂酰肌醇;PI(3)P：磷脂酰肌醇-3-磷酸;PI(4)P：磷脂酰肌醇-4-磷酸;PI(5)P：磷脂酰肌醇-5-磷酸;PI(3,4)P2：磷脂酰肌醇-3,4-二磷酸;PI(3,5)P2：磷脂酰肌醇-3,5-二磷酸酯;PI(4,5)P2：磷脂酰肌醇-4,5-二磷酸;PI(3,4,5)P3：磷脂酰肌醇-3,4,5-三磷酸;PS：磷脂酰丝氨酸;SM：鞘磷脂;S1P：鞘氨醇-1-磷酸;TAG：三酰甘油
Fig. 5. Both Ms1 and ThMs1 bind phospholipids in vitro
A, B: Purified MBP-His, MBP-Ms1-His and MBP-ThMs1-His; MBP-His, MBP-Ms1-His and MBP-ThMs1-His were overlaid in an Echelon P-6001 lipid strip (C) and Echelon P-6002 membrane strip (D). CL: Cardiolipin. Cs: Cholesterol. DAG: Diacylglycerol. LPA: Lysophosphatidic acid. LPC: Lysophos-phocholine; PC: Phosphatidylcholine; PE: Phosphatidylethanolamine; PG: Phosphatidylglycerol; PI: Phosphatidy-linositol; PI(3)P: Phosphatidylinositol-3-phosphate; PI(4)P: Phosphatidylinositol-4-phosphate; PI(5)P: Phosphatidylinositol-5-phosphate; PI(3,4)P2: Phosphatidylinositol-3,4-bisphosphate; PI(3,5)P2: Phospha- tidylinositol-3,5-bisphosphate; PI(4,5)P2: Phosphatidylinositol-4,5-bisphosphate; PI(3,4,5)P3: Phospha- tidylinositol-3,4,5-triphosphate; PS: Phosphatidylserine; SM: Sphingomyelin; S1P: Sphingosine-1 -phosphate; TAG: Triacylglycerol
