十倍体长穗偃麦草雄性育性基因ThMs1的克隆、表达及功能分析
衡燕芳1(
),李健2,王峥2,陈卓1,何航2,邓兴旺2,马力耕1()
),李健2,王峥2,陈卓1,何航2,邓兴旺2,马力耕1()
Cloning, Expression and Functional Analysis of a Male Fertility Gene ThMs1 in Bread Wheat
HENG YanFang1(),LI Jian2,WANG Zheng2,CHEN Zhuo1,HE Hang2,DENG XingWang2,MA LiGeng1()
),LI Jian2,WANG Zheng2,CHEN Zhuo1,HE Hang2,DENG XingWang2,MA LiGeng1()
图4. Ms1和ThMs1分别在普通小麦以及ms1g-4Ag附加系中的表达检测
A、B：半定量PCR;C、D：实时荧光定量PCR;E、F：RNA原位杂交分析。Ms1在普通小麦中检测,ThMs1在4Ag附加系中检测。Sp：造孢细胞;T：绒毡层;MMC：小孢子母细胞;MC：减数分裂细胞;Tds：四分体;Le&pa：内外稃;UM：单核期花粉;BP：双核期花粉;MP：三核期花粉;标尺=50 μm
Fig. 4. Expression analysis of ThMs1 and Ms1 in ms1g-4Ag addition line and bread wheat, respectively
A, B: Expression analysis by RT-PCR; C, D: quantitative RT-PCR; E, F: RNA in situ hybridization analysis. Ms1 in bread wheat and ThMs1in 4Ag addition line anthers. Sp: Sporogenous cell; T: Tapetum; MMC: Microspore mother cell; MC: Meiotic cell; Tds: Tetrads; Le&pa: Lemma and palea; UM: Unicellular microspore; BP: Bicellular pollen; MP: Mature pollen; Bar = 50 μm
