十倍体长穗偃麦草雄性育性基因ThMs1的克隆、表达及功能分析
衡燕芳1(
),李健2,王峥2,陈卓1,何航2,邓兴旺2,马力耕1()
),李健2,王峥2,陈卓1,何航2,邓兴旺2,马力耕1()
Cloning, Expression and Functional Analysis of a Male Fertility Gene ThMs1 in Bread Wheat
HENG YanFang1(),LI Jian2,WANG Zheng2,CHEN Zhuo1,HE Hang2,DENG XingWang2,MA LiGeng1()
),LI Jian2,WANG Zheng2,CHEN Zhuo1,HE Hang2,DENG XingWang2,MA LiGeng1()
图3. ThMs1转化小麦ms1的转基因功能互补分析
普通小麦、小麦雄性不育突变体ms1g、背景为ms1g纯合的T1代ThMs1转基因阳性株系。A：开花期小麦穗,标尺=1 cm;B：花丝伸长之后的花药,标尺=1 mm;C：开花后15 d的种子,标尺=1 mm;D：成熟花粉粒I2-KI染色,标尺=200 μm,B、C和D图下数字表示统计有此表型的株数及观察的总株数
Fig. 3. Complementation of ms1g by transformation with ThMs1
Ms1, ms1g, T1 transgenic line containing ThMs1 in a ms1g background. A: Spikes at flowering, Bar=1 cm; B: Anthers before filament elongation, Bar=1 mm; C: Seeds at 15 d after pollination, Bar=1 mm; D: I2-KI staining of mature pollen grains, Bar=200 μm, the numbers below the figure in B, C and D indicate the number of plants with the phenotype and the number of observed plants
