十倍体长穗偃麦草雄性育性基因<i>ThMs1</i>的克隆、表达及功能分析

十倍体长穗偃麦草雄性育性基因ThMs1的克隆、表达及功能分析

衡燕芳¹(

),李健²,王峥²,陈卓¹,何航²,邓兴旺²,马力耕¹(

)

Cloning, Expression and Functional Analysis of a Male Fertility Gene ThMs1 in Bread Wheat

HENG YanFang¹(

),LI Jian²,WANG Zheng²,CHEN Zhuo¹,HE Hang²,DENG XingWang²,MA LiGeng¹(

)

图1. 普通小麦、ms1g和ms1g/偃麦草4Ag染色体附加系的表型鉴定及细胞学分析
A：开花期小麦穗,标尺=1 cm;B：花丝伸长之后开裂的花药,标尺=1 mm;C：成熟花粉粒I₂-KI染色,标尺=200 μm;D：授粉后15 d的种子,标尺=1 mm,B、C和D图下数字表示统计有此表型的株数及观察的总株数;E：基因组原位杂交分析,红色荧光代表附加染色体;标尺=10 μm;F：花粉DAPI染色,Bar=50 μm

Fig. 1. Phenotypic characterization and cytological analysis of bread wheat, ms1g and ms1g/addition line
A: Spikes at flowering, Bar=1 cm; B: Anthers after filament elongation, Bar=1 mm; C: I₂-KI staining of mature pollen, Bar=200 μm; D: Seeds at 15 d after pollination, Bar=1 mm, the numbers below the figure in B, C and D indicate the number of plants with the phenotypes and the number of all observed plants; E: Genomic in situ hybridization (GISH) of somatic metaphase chromosomes, Bar=10 μm; F: Microspores from DAPI-stained, Bar=50 μm