十倍体长穗偃麦草雄性育性基因ThMs1的克隆、表达及功能分析

衡燕芳,李健,王峥,陈卓,何航,邓兴旺,马力耕

Cloning, Expression and Functional Analysis of a Male Fertility Gene ThMs1 in Bread Wheat

HENG YanFang,LI Jian,WANG Zheng,CHEN Zhuo,HE Hang,DENG XingWang,MA LiGeng

附表1 本研究所用引物

Table S1 The primers used in this study

引物 Primer 序列 Sequence (5'→3') 目的 Intention ThMs1-F AGATCCCGGCGCCTGCTGCTC ThMs1扩增引物 PCR primer of ThMs1 ThMs1-R CGCAGGAGCTGTAGAGCGTGAGGA BF TAGCCATCTTTGATCAATGAGC 4Ag染色体鉴定 Analysis of 4Ag chromosome BR TGATGAAAGAGCTAGGTGATAGTTG TF1 CGCCAGGGTTTTCCCAGTCACGAC 转基因鉴定 Analysis of transgenic lines TR1 TACAATGGCTAGTAGAGATTTC TF2 ATACGTTTCCTGCTACAGATTTGAGG TR2 AAGCAGTGCCGCCAGAGGATCAACGC TF3 TTTGCGTTCTGCTGATGATGTG TR3 CTCAATTGTCCTTTAGACCATGTCTAAC TaACTIN-QF TTCCGTTGCCCTGAGGTCC RT-PCR和实时荧光定量PCR RT-PCR and real time PCR TaACTIN-QR TGATCTTCATGCTGCTTGGTGC Ms1-QF ACATCATCCTCTGAGTCGCG Ms1-QR GACCACGCAAACACGTACG ThMs1-QF TCCCGGCGCCTGCTGCTC ThMs1-QR CGGCAGAGGCAGGGGACG Ms1-ISH-F aagcttGAGCGAGGGAGAGAGAGACC RNA原位杂交 In situ hybridization Ms1-ISH-F gaattcATCACATAGCATCAGTGGTTC ThMs1-ISH-F aagcttCTAGCGAGCGAGCGAGAGG ThMs1-ISH-R gaattcCAACTGGACGGACCATGGC ThMs1-SB-F1 TGAGATATACGGAGCGATTTAG Southern杂交 Southern blot ThMs1-SB-R1 AGCACGGCAAGCTTTTGCTCTG His-F aattcCATCATCATCATCATCACTGActgca MBP-His载体构建 Vector construction of MBP-His His-R gTCAGTGATGATGATGATGATGg Ms1s-His-F gagggaaggatttcagaattcCAGCCGGGGGCGCCGTGC MBP-Ms1-His载体构建Vector construction of MBP-Ms1-His Ms1s-His-R cagtgccaagcttgcctgcagTCAGTGATGATGATGATGATGGGCCGCCTTGGACGGCG ThMs1s-His-F gagggaaggatttcagaattcGCGTTCGGGCCGCAGC MBP-ThMs1-His载体构建 Vector construction of MBP-ThMs1-His ThMs1s-His-R cagtgccaagcttgcctgcagtcaGTGATGATGATGATGATGGAAGAAGGCCGCCTTGGACG