1-MCP和延迟冷藏对‘早红考密斯’梨货架期间品质 和软化相关基因表达的影响

程玉豆,张亚光,关军锋,冯云霄,何近刚

Effects of 1-MCP and Delayed Cold-Storage on Quality and Expression of Softening Related Genes in ‘Doyenne du Comice’ Pear During Shelf-Life

CHENG YuDou,ZHANG YaGuang,GUAN JunFeng,FENG YunXiao,HE JinGang

表1 果实软化相关基因qRT-PCR引物

Table 1 Primers of the fruit softening-related gene for qRT-PCR

基因 Gene 登录号 Access No. 正向引物 Forward primer 反向引物 Reverse primer PG1 KC855751 5′-GCTTATCCTTCATACATGGACACC-3′ 5′-CTGATGATGTTGCTATCCCACC-3′ PG2 KC855752 5′-AAATCAGAAAATCCTAAAACCAGC-3′ 5′-GCTTATAGCCATCATCAACAACG-3′ ARF1 KC855732 5′-CTTTTTTCCACAATCCTCATTCC-3′ 5′-GCTCTCGACGGTATAGAATTTGC-3′ ARF2 KC855733 5′-TAGCAACATTCCCATCCACC-3′ 5′-AACATTACACTGCCTATGATCTCG-3′ GAL4 KC855745 5′-GATCAATTCACTATCCCAGAAGC-3′ 5′-GTATAACATCCAAGCCTCCAGC-3′ ACT2 GU830958 5′-GGACATTCAACCCCTCGTCT-3′ 5’-ATCCTTCTGACCCATACCAACC-3’