无核葡萄离体胚珠发育影响因子及其生理变化
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李桂荣,全冉,程珊珊,侯小进,樊秀彩,扈惠灵
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The Influencing Factors of in-vitro Ovule Development in Seedless Grape and Its Physiological Changes
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LI GuiRong,QUAN Ran,CHENG ShanShan,HOU XiaoJin,FAN XiuCai,HU HuiLing
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表4 不同培养基和离体培养时间对离体培养胚珠可溶性蛋白含量的影响
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Table 4 Effects of different medium and culture time on soluble protein content of ovule in vitro (mg·g-1)
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培养基 Medium | 时间Time (d) | 42 | 49 | 56 | 63 | M1 | 0.28±0.04cde | 0.32±0.03def | 0.31±0.03cde | 0.18±0.04bcde | M2 | 0.30±0.01cd | 0.34±0.04de | 0.32±0.03bcd | 0.20±0.02abc | M3 | 0.33±0.04bc | 0.37±0.04bcd | 0.33±0.04bc | 0.22±0.04abc | M4 | 0.36±0.01b | 0.40±0.04bc | 0.36±0.03b | 0.22±0.03ab | M5 | 0.23±0.02fg | 0.31±0.05def | 0.3±0.01cde | 0.20±0.05abc | M6 | 0.26±0.02def | 0.36±0.02cd | 0.36±0.01b | 0.23±0.04ab | M7 | 0.35±0.03b | 0.44±0.03b | 0.43±0.03a | 0.26±0.04a | M8 | 0.46±0.03a | 0.49±0.03a | 0.47±0.05a | 0.26±0.05a | M9 | 0.19±0.02hi | 0.21±0.04h | 0.21±0.01gh | 0.18±0.04bcde | M10 | 0.21±0.02gh | 0.25±0.01gh | 0.24±0.01fg | 0.19±0.04abcd | M11 | 0.25±0.04efg | 0.27±0.03fg | 0.27±0.01ef | 0.21±0.04abc | M12 | 0.26±0.05defg | 0.28±0.05ef | 0.27±0.01def | 0.25±0.03ab | M13 | 0.11±0.02k | 0.13±0.02j | 0.13±0.03j | 0.09±0.04f | M14 | 0.12±0.02jk | 0.14±0.01ij | 0.14±0.04ij | 0.11±0.04ef | M15 | 0.14±0.02jk | 0.18±0.02hij | 0.17±0.04hi | 0.12±0.03def | M16 | 0.16±0.03jk | 0.19±0.03hi | 0.18±0.04hi | 0.14±0.05cdef |
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