无核葡萄离体胚珠发育影响因子及其生理变化

李桂荣,全冉,程珊珊,侯小进,樊秀彩,扈惠灵

The Influencing Factors of in-vitro Ovule Development in Seedless Grape and Its Physiological Changes

LI GuiRong,QUAN Ran,CHENG ShanShan,HOU XiaoJin,FAN XiuCai,HU HuiLing

表2 不同培养基和不同离体培养时间对离体培养胚珠发育率的影响

Table 2 Effects of different medium and culture time on ovule development rate in vitro (%)

培养基 Medium 时间Time (d) 42 49 56 63 M1 0.00±0.00f 1.10±1.91j 2.20±1.91d 1.10±1.91b M2 1.10±1.91ef 5.57±1.96ij 4.43±1.96cd 4.47±3.87ab M3 2.20±1.91ef 10.00±3.30hi 6.70±0.00cd 5.57±5.10ab M4 3.33±3.35def 14.43±1.96gh 15.57±1.96b 8.87±5.10a M5 4.47±3.87cdef 16.67±3.35f 24.43±1.96a 5.57±1.96ab M6 5.57±5.10cdef 25.57±1.96ef 26.67±3.35a 5.57±5.10ab M7 8.87±5.10abcdef 32.20±1.91cd 28.90±3.81a 6.70±0.00ab M8 17.77±13.85a 42.23±6.93a 30.00±3.30a 8.90±1.91a M9 7.77±5.08bcde 23.33±3.35e 25.57±1.96a 7.77±5.08ab M10 12.23±5.08abcd 30.00±3.30de 26.67±3.35a 7.77±5.08ab M11 13.33±3.35abc 36.67±3.35bc 28.90±3.81a 8.90±3.81a M12 16.67±3.35bc 40.00±3.30ab 28.90±3.81a 8.90±3.81a M13 3.33±3.35def 7.80±1.91i 4.47±3.87cd 3.33±3.35ab M14 4.47±3.87cdef 8.90±1.91hi 5.57±5.10cd 4.47±3.87ab M15 7.80±1.91bcde 8.90±1.91hi 8.87±5.10c 4.47±3.87ab M16 10.00±0.00abcde 10.00±3.30hi 10.00±0.00c 6.67±3.35ab