无核葡萄离体胚珠发育影响因子及其生理变化

李桂荣,全冉,程珊珊,侯小进,樊秀彩,扈惠灵

The Influencing Factors of in-vitro Ovule Development in Seedless Grape and Its Physiological Changes

LI GuiRong,QUAN Ran,CHENG ShanShan,HOU XiaoJin,FAN XiuCai,HU HuiLing

表1 不同培养基处理

Table 1 Different medium treatments

编号 NO. 基本培养基 Basic medium 蔗糖 Sugar (g·L-1) M1 MS固体培养基MS solid medium 30 M2 MM3固体培养基MM3 solid medium 30 M3 改良MM3固体培养基Modified MM3 solid medium 30 M4 固液双层培养基（改良MM3固体培养基+8 mg·L-1 ER液体培养基） Solid-liquid double-layer medium (modified MM3 solid medium + 8mg·L-1 ER liquid medium) 30 M5 MS固体培养基MS solid medium 45 M6 MM3固体培养基MM3 solid medium 45 M7 改良MM3固体培养基Modified MM3 solid medium 45 M8 固液双层培养基（改良MM3固体培养基+8 mg·L-1 ER液体培养基） Solid-liquid double-layer medium (modified MM3 solid medium + 8mg·L-1 ER liquid medium) 45 M9 MS固体培养基MS solid medium 60 M10 MM3固体培养基MM3 solid medium 60 M11 改良MM3固体培养基Modified MM3 solid medium 60 M12 固液双层培养基（改良MM3固体培养基+8nmg·L-1 ER液体培养基） Solid-liquid double-layer medium (modified MM3 solid medium + 8 mg·L-1 ER liquid medium) 60 M13 MS固体培养基 MS solid medium 75 M14 MM3固体培养基 MM3 solid medium 75 M15 改良MM3固体培养基Modified MM3 solid medium 75 M16 固液双层培养基（改良MM3固体培养基+8 mg·L-1 ER液体培养基） Solid-liquid double-layer medium (modified MM3 solid medium + 8 mg·L-1 ER liquid medium) 75