红橘响应褐斑病菌侵染的转录组学分析
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唐科志,周常勇
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Transcriptome Analysis of Citrus reticulata Blanco, cv. Hongjv Infected with Alternaria alternata Tangerine Pathotype
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TANG KeZhi,ZHOU ChangYong
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表1 qRT-PCR验证基因选择及引物设计
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Table 1 Genes selection and primers design for qRT-PCR
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| 基因ID Gene ID | 引物序列 Primer sequence (5′-3′) | 产物长度 Length of product (bp) | | Cs4g12760 | F: TTCGTCTTGCTCTTCGGATAA | R: GCACTCCAACGGAATCTCTAA | 202 | | Cs2g09310 | F: GGTGTCATTCCTCCTCCTACC | R: GCAGTTCCCTCGCCTATTCT | 198 | | Cs5g15470 | F: CAGCCTTGTCGGTATGAGAA | R: CAACACCCAATCTTCCTTGAG | 151 | | Cs5g21860 | F: GCTCTTGTGGGCATTCTTGC | R: CTCTCGTGTAGAAGCTCTTGCC | 142 | | Cs7g20700 | F: ACGGTTCAGGCTCATTTCAG | R: TGGGATTTGGCATCATCAAT | 185 | | Cs3g10430 | F: TGGAGACGTAGAGGCTGTCAA | R: CATACCAATATTTTGAGCATTTT | 121 | | Cs6g07410 | F: GGAGAGTGGTGGAATGCTGAT | R: CACTTCGAGCGTGTAGGTTTG | 150 | | Cs6g20850 | F: CCAGCAGGCATGAGAAATTA | R: TGACCATCGTGGGAACAGTA | 229 | | Cs1g01180 | F: TGCAGTAGAAGTTGATGGTGATG | R: AATGAGCCGTTAGCGACAAG | 165 | | Cs1g04680 | F: AGCTGCAAGGGTTTGGTTAG | R: GAATTTGCGTTTGGTGATGA | 150 | | Cs8g13680 | F: GCCTATGCTTGCTGTTGTTTC | R: GAAGGCAGTCCATCCATACTTC | 194 | | orange1.1t03118 | F: CAAGCTCTCCAGGCAAGAGT | R: GGTCCACGGCCATAGTAGAA | 247 | | orange1.1t03618 | F: CGGGATGAACATTTGGTTTA | R: CTAGCCTTCTGATCTTGACACA | 184 | | orange1.1t05311 | F: GCTGGGATATAACTCCTTCTCA | R: TTCCGCTAAACCAATCACTT | 172 | | Cs2g06120 | F: GCACAAGGAAATGGGTTTGT | R: GAAACACGCTGGGATCACTT | 229 | | Cs6g07400 | F: ACATGGCTGCAAGAGCATAC | R: CCATTGAGGTCCACCACTTA | 197 | | orange1.1t00214 | F: ACGCTCTGTCCCTCAACAAG | R: CCGCTACTGCCTCCTGTATC | 171 | | orange1.1t02319 | F: GGGATCTACTGCCGACACTC | R: CGACGACGACCTTTGATCTT | 245 | | orange1.1t03603 | F: GGACAATGCTGATCCGAAAG | R: TCAACCAAGCCTCCTGAAAC | 203 | | CitActin | F: CATCCCTCAGCACCTTCC | R: CCAACCTTAGCACTTCTCC | 191 |
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