低温诱导稻曲病菌菌核形成的转录组学分析
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吕楚阳,邓平川,张晓丽,孙钰超,梁五生,胡东维
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Transcriptomic Analysis of Sclerotia Formation Induced by Low Temperature in Villosiclava virens
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LÜ ChuYang,DENG PingChuan,ZHANG XiaoLi,SUN YuChao,LIANG WuSheng,HU DongWei
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表1 实时荧光定量PCR所用引物
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Table 1 Primers used in qRT-PCR
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| 序号No. | 蛋白名称
Protein name | 蛋白ID
Protein ID | 引物序列
Primer sequence (5′-3′) | 扩增长度
Amplicon size (bp) |
|---|
| 1 | Protein rds1 | KDB11131.1 | F: TCCAACGTGCGGGAATACA | 164 | | R: CCAAACTGGCGGAAAATC | | 2 | Biotrophy-associated secreted protein 2 | KDB17523.1 | F: GCCTCAGCAACACCGACT | 220 | | R: GCCTTTACCTCGTCCGCC | | 3 | Glycoside hydrolase | KDB17641.1 | F: TCCTCGCCACCATCTCGT | 178 | | R: CGCCTCATCGCCCTCAAC | | 4 | Cytosolic phospholipase A2 zeta | KDB15918.1 | F: ATGGGCGTCTTTGGGAGCG | 165 | | R: GGGAATTGTGGCGGGATCT | | 5 | CRE-TRX-1 protein | KDB18233.1 | F: GCCAAATCCCGACAAAAG | 142 | | R: GGCGGCGTAGTCACCATA | | 6 | Methylenetetrahydrofolate reductase 1 | KDB13169.1 | F: TCCTCGCCACCATCTCGTC | 179 | | R: CCGCCTCATCGCCCTCAAC | | 7 | α-tubulin | KDB12764.1 | F: GCTCTCGTGCTTGCTCTTGG | 144 | | R: ATCACTTCGTCCTTGCGTTT |
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