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陆地棉GhWRKY33的克隆及抗旱功能分析
魏鑫1,王寒涛2,魏恒玲2,付小康2,马亮2,芦建华2,王省芬1(),喻树迅2()
Cloning and Drought Resistance Analysis of GhWRKY33 in Upland Cotton
WEI Xin1,WANG HanTao2,WEI HengLing2,FU XiaoKang2,MA Liang2,LU JianHua2,WANG XingFen1(),YU ShuXun2()

图3. GhWRKY33与其他物种WRKY蛋白序列比对分析
方框表示WRKY结构域,星号表示锌指结构。拟南芥AtWRKY33(NP_181381)、雷蒙德氏棉GrWRKY33(XP_012460140)、亚洲棉GaWRKY33(XP_017615740)、可可TcWRKY33(XP_017977471)、哥伦比亚锦葵HuWRKY33(XP_021290063)、榴莲DzWRKY33(XP_022767833)、黄麻CcWRKY33(OMO85052)、番木瓜CpWRKY33(XP_021908634)、橡胶树HbWRKY33(XP_021664002)、银白杨PaWRKY17(TKS07377)、木薯MeWRKY33(XP_021623467)、麻风树JcWRKY33(XP_012089749)、龙眼DlWRKY17(AEO31478)、橄榄树CaWRKY33(AXY96406)、山胡椒LgWRKY33(ALE71299)、豇豆VuWRKY33(QCE01025)、杨梅MrWRKY33(KAB1225722)、葡萄VvWRKY24(XP_002272040)、大豆GmWRKY49(NP_001304523)、大豆GmWRKY39(NP_001348302)、棉花GhWRKY33(XP_016680843)。下同

Fig. 3. Sequence alignment of GhWRKY33 and WRKY proteins of other species The box indicates the WRKY domain, and the star indicates the zinc finger structure.
Arabidopsis thaliana AtWRKY33 (NP_181381), Gossypium raimondii GrWRKY33 (XP_012460140), Gossypium arboretum GaWRKY33 (XP_017615740), Theobroma cacao TcWRKY33 (XP_017977471), Herrania umbratica HuWRKY33 (XP_021290063), Durio zibethinus DzWRKY33 (XP_022767833), Corchorus capsularis CcWRKY33 (OMO85052), Carica papaya CpWRKY33 (XP_021908634), Hevea brasiliensis HbWRKY33 (XP_021664002), Populus alba PaWRKY17 (TKS07377), Manihot esculenta MeWRKY33 (XP_021623467), Jatropha curcas JcWRKY33 (XP_012089749), Dimocarpus longan DlWRKY17 (AEO31478), Canarium album CaWRKY33 (AXY96406), Lindera glauca LgWRKY33 (ALE71299), Vigna unguiculata VuWRKY33 (QCE01025), Morella rubra MrWRKY33 (KAB1225722), Vitis vinifera VvWRKY33 (XP_002272040), Glycine max GmWRKY49 (NP_001304523), Glycine max GmWRKY39 (NP_001348302), Gossypium hirsutum GhWRKY33 (XP_016680843). The same as below