陆地棉GhWRKY33的克隆及抗旱功能分析

魏鑫,王寒涛,魏恒玲,付小康,马亮,芦建华,王省芬,喻树迅

Cloning and Drought Resistance Analysis of GhWRKY33 in Upland Cotton

WEI Xin,WANG HanTao,WEI HengLing,FU XiaoKang,MA Liang,LU JianHua,WANG XingFen,YU ShuXun

表1 研究所用引物

Table 1 Primers used in this study

引物名称 Primer name 引物序列 Primer sequence（5′-3′） GhWRKY33-F CACGGGGGACTCTAGAATGGCTGCTTCATCATCATCT GhWRKY33-R GACGGCCAGTGAATTCTCAAGACAGGAATCCGTCCAA qRT-GhWRKY33-F GGGAAACCCCAATCCAAGGAGC qRT-GhWRKY33-R AATCATGGGATGCTCGCTCCAC qRT-GhActin-F ATCCTCCGTCTTGACCTTG qRT-GhActin-R TGTCCGTCAGGCAACTCAT GhWRKY33-GFP-F CACGGGGGACTCTAGAATGGCTGCTTCATCATCATCT GhWRKY33-GFP-R CTTTACTCATACTAGTAGACAGGAATCCGTCCAAAAATG qRT-AtRD29A-F ATCACTTGGCTCCACTGTTGTTC qRT-AtRD29A-R AAAACACACATAAACATCCAAAGT qRT-AtCOR15A-F ACTCAGTTCGTCGTCGTTTCT qRT-AtCOR15A-R CTTCTTTTCCTTTCTCCTCCAC qRT-AtP5CS-F GAGGGGGTATGACTGCAAAA qRT-AtP5CS-R AACAGGAACGCCACCATAAG qRT-AtUBQ1-F TGAGCCTTCCTTGATGATGCT qRT-AtUBQ1-R GCACTTGCGGCAAATCATCT