基于粗提物的水稻条纹病毒体外“抓帽”体系
林文忠(
),吴然,金晶,丘萍,张洁,吴祖建(),杜振国()
),吴然,金晶,丘萍,张洁,吴祖建(),杜振国()
An in vitro Cap-Snatching System of Rice Stripe Tenuivirus Based on Crude Virion Preparations
LIN WenZhong(),WU Ran,JIN Jing,QIU Ping,ZHANG Jie,WU ZuJian(),DU ZhenGuo()
),WU Ran,JIN Jing,QIU Ping,ZHANG Jie,WU ZuJian(),DU ZhenGuo()
图1. 利用巢式RT-PCR扩增RSV从珠蛋白-α“抓帽”后合成的NP（转录自vRNA3）和NCP（转录自vcRNA4）mRNA
Fig. 1. Schematic diagram showing the nested RT-PCR used to detect RSV NP (transcribed from vRNA3) and NCP (transcribed from vcRNA4) mRNAs with capped-RNA leaders obtained from globin-α mRNA
