基于粗提物的水稻条纹病毒体外“抓帽”体系
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林文忠,吴然,金晶,丘萍,张洁,吴祖建,杜振国
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An in vitro Cap-Snatching System of Rice Stripe Tenuivirus Based on Crude Virion Preparations
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LIN WenZhong,WU Ran,JIN Jing,QIU Ping,ZHANG Jie,WU ZuJian,DU ZhenGuo
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表2 RSV从珠蛋白-α mRNA“抓帽”后生成的NP mRNA
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Table 2 RSV NP mRNAs transcribed using capped-RNA leaders snatched from globin-α mRNA as primers
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行数
Row | 珠蛋白-α mRNA序列Globin-α mRNA sequence
m7G-ACACUUCUGGUCCAGUCCGACUGAG- | 克隆数
Clone number | 引发与重配
Priming and realignment | | 1 | m7G-ACACUUCUGGUC12------------ACACAAAGUC- | 1 | + | | 2 | m7G-ACACUUCUGGUCCAGUCC18------ACACAAAGUC- | 7 | + | | 3 | m7G-ACACUUCUGGUC12-----AC-----ACACAAAGUC- | 1 | + | | 4 | m7G-ACACUUCUGGUC12----ACAC----ACACAAAGUC- | 1 | + | | 5 | m7G-ACACUUCUGGUC12-ACACACACAC-ACACAAAGUC- | 3 | + | | 6 | m7G-ACACUUCUGGUCCA14----------CACAAAGUC- | 5 | - | | 7 | m7G-ACACUUCUGGUCCA14------------CAAAGUC- | 1 | - |
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