基于粗提物的水稻条纹病毒体外“抓帽”体系

林文忠,吴然,金晶,丘萍,张洁,吴祖建,杜振国

An in vitro Cap-Snatching System of Rice Stripe Tenuivirus Based on Crude Virion Preparations

LIN WenZhong,WU Ran,JIN Jing,QIU Ping,ZHANG Jie,WU ZuJian,DU ZhenGuo

表1 本研究所用的主要试剂和试剂盒

Table 1 The reagents and kits used in this study

试剂名称 Reagents and kits 来源 Manufacture 用途 Usage RCL（未经核酸酶处理 Not treated with nuclease） Promega 体外“抓帽”体系 In vitro cap-snatching RNase inhibitor Promega NTP (100 mmol·L-1 each) TaKaRa m7G (5′) ppp (5′) G NEB Prestained Protein Marker GenStar SDS-PAGE RNA purification Kit (DP412) TIANGEN RSV mRNA的检测与克隆 Detection and cloning of RSV mRNAs 1st Strand cDNA Synthesis Kit Vazyme Biotech 2×ExTaq Mix TaKaRa HiPure Gel Pure DNA Mini Kit Magen pMD19-T TaKaRa