图9. 潜在调控BOLL的非编码RNAs的时间表达模式及靶向关系验证
A:RNA-Seq（4个生物学重复）和qRT-PCR验证（8个生物学重复组成,每个由3个技术重复组成）获得的非编码RNA表达模式的比较。RNA-seq获得的miRNAs、circRNAs和lncRNAs的表达丰度分别用TPM（每千个碱基的转录每百万映射读取的转录本数）、RPM（每百万映射读取的reads数）和FPKM（每千个碱基的转录每百万映射读取的片段数）衡量。B:双荧光素酶报告法验证miRNAs与BOLL或其ceRNAs的靶向关系。3M:3月龄;1Y:1岁龄;3Y:3岁龄。**:差异极显著（P<0.01）;*:差异显著（P<0.05）;ns:差异不显著（P>0.05）

Fig. 9. Temporal expression patterns of potential regulatory non-coding RNAs for BOLL gene and verification of the target relationship
A: Comparison of the changes in expression of non-coding RNAs obtained by RNA-seq (four biological replicates) and by qRT-PCR validation (eight biological replicates each consisting of three technical replications). The expression abundance of miRNAs, circRNAs, and lncRNAs obtained from RNA-seq was measured by the TPM (Transcripts Per Kilobase of exon model per Million mapped reads), RPM (Reads of exon model per Million mapped reads), and FPKM (Fragments Per Kilobase of exon model per Million mapped fragments), respectively. B: A dual luciferase reporter assay was used to validate the targeting relationships between miRNAs and BOLL or their ceRNAs. 3M: 3-month-old; 1Y: 1-year-old; 3Y: 3-year-old. **: Extremely significant difference (P<0.01); *: Significant difference (P<0.05); ns: Non-significant difference (P>0.05)