图6. 发育的藏绵羊睾丸中BOLL mRNA和蛋白的表达规律
A:RNA-Seq和qRT-PCR验证获得的BOLL mRNA表达变化规律的比较。所有RNA-seq试验由4个生物学重复组成;所有qRT-PCR试验由8个生物学重复组成,每个生物学重复由3技术重复组成。RNA-seq获得的BOLL mRNA表达丰度用FPKM值（每千个碱基的转录每百万映射读取的片段数）表示。B:BOLL 蛋白的Western blot分析;C:BOLL蛋白相对表达量。3M:3月龄;1Y:1岁龄;3Y:3岁龄。**:差异极显著（P<0.01）;ns:差异不显著（P>0.05）

Fig. 6. Expression patterns of BOLL mRNA and protein in developmental Tibetan sheep testes
A: Comparison of the changes in mRNA expression of BOLL acquired by RNA-seq and by qRT-PCR validation. All RNA-seq experiments consisted of four biological replicates; all qRT-PCR experiments consisted of eight biological replicates each consisting of three technical replications. The mRNA expression abundance of BOLL obtained from RNA-seq was expressed by the FPKM (Fragments Per Kilobase of exon model per Million mapped fragments) value. B: Western blot analysis for BOLL protein; C: The relative level of BOLL protein expression. 3M: 3-month-old; 1Y: 1-year-old; 3Y: 3-year-old. **: Extremely significant difference (P<0.01); ns: Non-significant difference (P>0.05)