藏绵羊BOLL的分子特征及其在睾丸中的表达调控与功能分析
李讨讨1(
),王霞1,马友记1,2(),尹德恩1,张勇3,赵兴绪3
),王霞1,马友记1,2(),尹德恩1,张勇3,赵兴绪3
Molecular Characterization of Tibetan Sheep BOLL and Its Expression Regulation and Functional Analysis in Testis
LI TaoTao1(),WANG Xia1,MA YouJi1,2(),YIN DeEn1,ZHANG Yong3,ZHAO XingXu3
),WANG Xia1,MA YouJi1,2(),YIN DeEn1,ZHANG Yong3,ZHAO XingXu3
图1. 藏绵羊BOLL CDS区克隆及序列分析
A:绵羊BOLL结构图（序列数据来源于GenBank:accession No. NC_040253.1）。B:藏绵羊BOLL cDNA的PCR扩增。M,DL2000分子标记。C:BOLL cDNA扩增产物的开放阅读框（ORF）分析。D:克隆的BOLL CDS区核苷酸序列及推导的氨基酸序列。ATG和TAA分别为起始密码子和终止密码子
Fig. 1. Cloning and sequence analysis of Tibetan sheep BOLL CDS region
A: Structural diagram of the ovine BOLL (The sequence data is available from GenBank: accession No. NC_040253.1). B: PCR amplification derived from cDNA of Tibetan sheep BOLL gene. M, DL2000 marker. C: Open reading frame (ORF) analysis of Tibetan sheep BOLL cDNA amplification product. D: The nucleotide and deduced amino acid sequences for the cloned BOLL CDS region. ATG and TAA are the start codon and the stop codon, respectively
