PSMB5蛋白对牛骨骼肌卫星细胞增殖与成肌分化的影响
来裕婷(),朱菲菲,王轶敏,郭宏,张林林,李新,郭益文,丁向彬()
Effects of PSMB5 on the Proliferation and Myogenic Differentiation of Skeletal Muscle Satellite Cells
LAI YuTing(),ZHU FeiFei,WANG YiMin,GUO Hong,ZHANG LinLin,LI Xin,GUO YiWen,DING XiangBin()

图5. 干扰和过表达PSMB5对牛骨骼肌卫星细胞分化的影响
(A-B)转染siRNA-PSMB5和pcDNA-PSMB5及对照组牛骨骼肌卫星细胞诱导成肌分化的效果(100×);(C-D)Western blotting检测干扰PSMB5对分化标志因子MyHC及PSMB5蛋白表达水平的影响 (E-F) Western blotting检测过表达PSMB5对分化标志因子MyHC及PSMB5的蛋白表达水平的影响

Fig. 5. Effects of interference and overexpression of PSMB5 on the differentiation of bovine skeletal muscle satellite cells
Comment: (A-B)Effect of transfection of siRNA-PSMB5 and pcDNA-PSMB5 and control group bovine skeletal muscle satellite cells on myogenic differentiation (100×)(C-D)Western blotting to detect the effect of interfering with PSMB5 on the expression levels of PSMB5 and differentiation markers MyHC(E-F)Western blotting to detect the effect of over-expressing PSMB5 on the protein expression levels of PSMB5 and differentiation markers MyHC