PSMB5蛋白对牛骨骼肌卫星细胞增殖与成肌分化的影响

来裕婷,朱菲菲,王轶敏,郭宏,张林林,李新,郭益文,丁向彬

Effects of PSMB5 on the Proliferation and Myogenic Differentiation of Skeletal Muscle Satellite Cells

LAI YuTing,ZHU FeiFei,WANG YiMin,GUO Hong,ZHANG LinLin,LI Xin,GUO YiWen,DING XiangBin

表1 qRT-PCR引物信息

Table 1 The primer information of qRT-PCR

引物名称 Primer name 引物序列 Primer sequence 片段长度 Fragment length PSMB5-F 5′-TCAAGATGTCAGCCACACCC -3′ 112 PSMB5-R 5′-ACCACGCCTCCATACACAAG -3′ MyHC-F 5′- CTGGAATCCGGAGGCAGAA-3′ 105 MyHC-R 5′- TTTTCGAAGGTAGGGAGCGG-3′ GAPDH-F 5′- TGTTGTGGATCTGACCTGCC-3′ 135 GAPDH-R 5′- AAGTCGCAGGAGACAACCTG-3′