龙眼SPL基因家族全基因组鉴定及表达分析

路保顺,朱永静,张舒婷,吕煜梦,李晓斐,宋雨洋,赖钟雄,林玉玲

Whole-Genome Identification and Expression Analysis of SPL Gene Family in Dimocarpus Longan

LU BaoShun,ZHU YongJing,ZHANG ShuTing,LÜ YuMeng,LI XiaoFei,SONG YuYang,LAI ZhongXiong,LIN YuLing

表1 qRT-PCR引物序列信息

Table 1 qRT-PCR primers of DlSPL in this study

基因名称 Gene name 引物序列 Primer sequence 退火温度 Annealing temperature (℃) 产物大小 Product size (bp) DlSPL1 DlSPL5 DlSPL7 DlSPL8 DlSPL13 DlSPL14 EF-1α CCCACGTGTGTTTACCGAG GGAGACAGATCCTTGATTGGTT GGTACCCATCCCTTTACGG TCGGAGTTTGATGAGGCC TGGTCAAACACAACAGAGCC AGGCCCCAAAGCTTCACTAT GAGGAGTGGTCTTCGAAGGAT TGTTGGAGTACGAGTGGGC GCAGCTTCTCTTTCCCTCATC AGTCCCTTCTTGCTTGGTTG GCTTCTCTTTCCCTCGTCAA ACCGCCGTCATAAAGTGTGT GATGATTCCCACCAAGCCCAT GGGTCCTTCTTCTCAACACTCT 60.0 59.4 59.1 59.7 59.7 60.1 59.7 59.7 60.5 59.3 59.6 60.4 61.3 58.9 113 122 196 144 175 133 129