耐草甘膦转EPSPS/GAT大豆多重PCR检测体系的建立及应用
文静1,2(
),郭勇2,邱丽娟1,2
),郭勇2,邱丽娟1,2
Establishment and Application of Multiple PCR Detection System for Glyphosate-Tolerant Gene EPSPS/GAT in Soybean
WEN Jing1,2(),GUO Yong2,QIU LiJuan1,2
),GUO Yong2,QIU LiJuan1,2
图4. 单重及多重PCR检测ZH10-6及中黄10
M:2K Plus II;1:中黄10;2:ZH10-6;GmActin11、G2-EPSPS、GAT、ZH10P1/GAT、G2/ZH10P2为PCR扩增引物,5 Pairs of Primers是GmActin11、G2-EPSPS、GAT、ZH10P1/GAT、G2/ZH10P2等量混合
Fig. 4. Single and multiple PCR detection of ZH10-6 and ZH10
M: 2K Plus II; 1: ZH10; 2: ZH10-6; GmActin11, G2-EPSPSP, GAT, ZH10P1/GAT and G2/ZH10P2 were PCR amplification primers, and 5 Pairs of Primers were equal combinations of GmActin11, G2-EPSPSP, GAT, ZH10P1/GAT and G2/ZH10P2
