耐草甘膦转<i>EPSPS</i>/<i>GAT</i>大豆多重PCR检测体系的建立及应用

耐草甘膦转EPSPS/GAT大豆多重PCR检测体系的建立及应用

文静,郭勇,邱丽娟

Establishment and Application of Multiple PCR Detection System for Glyphosate-Tolerant Gene EPSPS/GAT in Soybean

WEN Jing,GUO Yong,QIU LiJuan

表1 多重PCR标记及其相关信息

Table 1 The markers for multiplex PCR and their related information

基因/标记名称 Gene/Marker name	引物名称 Primer name	引物序列 Primer sequence (5′-3′)	产物大小 Fragment size (bp)
内源参考基因Actin Endogenous gene Actin	GmActin11F	ATCTTGACTGAGCGTGGTTATTCC	126
内源参考基因Actin Endogenous gene Actin	GmActin11R	GCTGGTCCTGGCTGTCTCC	126
侧翼序列ZH10P1/GAT-2 Flanking sequence ZH10P1/GAT-2	ZH10P1	TAATAGTAGAATGGGACTGGTGGAT	810
侧翼序列ZH10P1/GAT-2 Flanking sequence ZH10P1/GAT-2	GAT	GCGGACTTGCTTTGGTGTAAT	810
侧翼序列G2EPSPS-2/ZH10P2 Flanking sequence G2EPSPS-2/ZH10P2	G2	CCCGAATCATCAGGCAAACA	1626
侧翼序列G2EPSPS-2/ZH10P2 Flanking sequence G2EPSPS-2/ZH10P2	ZH10P2	AACACATCATAGTATTCTAAAACGCTT	1626
外源基因G2-EPSPS Exogenous gene G2-EPSPS	G2-EPSPS F	CAAATCCATTACCAACCGTGC	430
外源基因G2-EPSPS Exogenous gene G2-EPSPS	G2-EPSPS R	ACCACCATCAATCTCGAAACG	430
外源基因GAT Exogenous gene GAT	GAT F	CTCAGACCAAACCAGCCGATAG	338
外源基因GAT Exogenous gene GAT	GAT R	GTGTCGAATACCTCTCCCTGCTC	338