人工合成小麦SHW-L1高硒含量KASP分子标记开发及其应用

魏广辉,李执,陈强,李阳,陈诗豪,裴英,周勇,程梦萍,唐豪,王际睿,魏育明,刘登才,陈黎,郑有良,蒲至恩

Development and Utilization of KASP Marker for Se Concentration in Synthetic Wheat SHW-L1

WEI GuangHui,LI Zhi,CHEN Qiang,LI Yang,CHEN ShiHao,PEI Ying,ZHOU Yong,CHENG MengPing,TANG Hao,WANG JiRui,WEI YuMing,LIU DengCai,CHEN Li,ZHENG YouLiang,PU ZhiEn

表3 所用SNP标记

Table 3 SNP markers used in this study

编号 Number 染色体 Chromosome 变异碱基 Base 序列 Sequence (5′-3′) AX-1 5A A/G CGTACCATGCGATCACACTTGGAGATGCGTGTGAG[A/G]GAAAAAGAGTACTCCATCCATCCAAATTCCATCCC AX-2 5A A/G TGTAAATTAATTTGTTGTGGGTAGGGCATCTTGAG[A/G]AATGTTTACCTTCTTGCTGCTGCCTGGTTCGGTTT AX-3 5A A/C GGGTGATAGCAAGGAAGCTGAAGAGCATGATGGAC[A/C]AGGGACAGACGGAAAAGAGTTGCAGGATGGACAAG AX-4 5A A/G GATGACTTTGCGGCAATATTAAGAGATGTGCATCC[A/G]TTAGCAAGCAAGCTAGCGGACAAAACCAGCTTAAC AX-5 5A T/C ACCCCTTGTTTAGTACCACCTCACCAAGTGAGAGT[C/T]ATTTCAACCGGTTTATAAGATTCAGCGTTCCAACC