代乳粉水平对羔羊血液指标和肠道屏障功能的影响
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李彦军,牛骁麟,张千,王国秀,李发弟,李飞,李冲,庞鑫,贾莉,樊海苗
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Effects of Milk Replacer Feeding Level on Hematology Index and Gut Barrier Function in Lambs
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YanJun LI,XiaoLin NIU,Qian ZHANG,GuoXiu WANG,FaDi LI,Fei LI,Chong LI,Xin PANG,Li JIA,HaiMiao FAN
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表2 荧光定量PCR引物序列
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Table 2 Sequences of forward and reverse primers used for real-time PCR
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基因
Gene | 检索号
Gene bank number | 引物序列
Primer sequence | 片段长度
Size (bp) | 扩增效率
Amplification efficiency | | Claudin-1 | NM_001185016.1 | F: 5' AATACATTGAGGTCACCGAGTA 3' | 191 | 98.93% | | | R: 5' GATTAGGCAAGGAAAGGCAC 3' | | | | Claudin-4 | NM_001185017.1 | F: 5' GCCTTCATCGGCAGCAACAT 3' | 115 | 92.75% | | | R: 5' CCAGCAGCGAGTCGTACACCTT 3' | | | | Occludin | XM_012145891.2 | F: 5' AGTGGTAACTTGGAGACGCTTTC 3' | 107 | 95.17% | | | R: 5' CCTCCCGTCGTGTAGTCTGTT 3' | | | | TLR4 | NM_001135930.1 | F: 5' GGTTTCAGGAATGCCACTT 3' | 142 | 98.14% | | | R: 5' CTTTCACCTCTGCCATACTTT 3' | | | | TNFα | NM_001114186.1 | F: 5' ACGGCGTGGAGCTGAAA 3' | 132 | 92.18% | | | R: 5' CTGATGGTGTGGGTGAGGAA 3' | | | | β-Actin | NM_001009784.2 | F: 5' TCCGTGACATCAAGGAGAAGC 3' | 266 | 91.82% | | | R: 5' CCGTGTTGGCGTAGAGGT 3' | | |
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