紫花苜蓿MsSQE1的克隆及对皂甙合成的功能分析
康俊梅,张俏燕,蒋旭,王珍,张铁军,龙瑞才,崔会婷,杨青川()
Cloning MsSQE1 from Alfalfa and Functional Analysis in Saponin Synthesis
KANG JunMei,ZHANG QiaoYan,JIANG Xu,WANG Zhen,ZHANG TieJun,LONG RuiCai,CUI HuiTing,YANG QingChuan()

图9. 转基因苜蓿的鉴定及过表达MsSQE1对苜蓿总皂甙含量的影响
A：阳性转基因植株鉴定;B：qRT-PCR测定MsSQE1的相对表达量;C：转基因植株总皂甙含量测定;CK+：pBI-121-MsSQE1质粒（阳性对照）;CK-：转空载体pBI-121苜蓿植株（阴性对照）;1—10：抗性转化植株;1、9、10：过表达MsSQE1的转基因紫花苜蓿

Fig. 9. Identification of transgenic alfalfa and effect of overexpression MsSQE1 on total saponin content of alfalfa
A: Verification of positive transgenic plants; B: Analysis of the relative expression level of MsSQS by qRT-PCR in the three representative transgenic lines; C: Content of total saponins in the indicated transgenic lines. CK+: Plasmid harboring 35S::MsSQS (positive control); CK-: pBI-121 empty vector (negative control); 1-10: Regenerated alfalfa plants resistant to kanamycin; 1,9,10: Three independent transgenic alfalfa lines. Bars represent the mean ± SD of three biological replicates