紫花苜蓿MsSQE1的克隆及对皂甙合成的功能分析
康俊梅,张俏燕,蒋旭,王珍,张铁军,龙瑞才,崔会婷,杨青川()
Cloning MsSQE1 from Alfalfa and Functional Analysis in Saponin Synthesis
KANG JunMei,ZHANG QiaoYan,JIANG Xu,WANG Zhen,ZHANG TieJun,LONG RuiCai,CUI HuiTing,YANG QingChuan()

图6. SDS-PAGE分析大肠杆菌中紫花苜蓿鲨烯环氧酶的表达
M：蛋白分子质量标准;1：转化了pEASY-E2-Control的大肠杆菌总蛋白（经过IPTG诱导表达5 h）;2：转化了pEASY-E2-MsSQE1ΔN25的大肠杆菌总蛋白（未经IPTG诱导表达）;3：转化了pEASY-E2-MsSQE1ΔN25大肠杆菌上清蛋白（经过IPTG诱导表达5 h）;4：转化了pEASY-E2-MsSQE1ΔN25大肠杆菌沉淀蛋白（经过IPTG诱导表达5 h）;5：转化了pEASY-E2-MsSQE1的大肠杆菌总蛋白（未经IPTG诱导表达）;6：转化了pEASY-E2- MsSQE1大肠杆菌上清蛋白（经过IPTG诱导表达5 h）;7：转化了pEASY-E2-MsSQE1大肠杆菌沉淀蛋白（经过IPTG诱导表达5 h）;箭头表示MsSQE1蛋白的表达

Fig. 6. Expression analysis of MsSQE1/MsSQE1ΔN25 by SDS-PAGE in E.coli
M: Protein ladder; 1: Total protein expressed by E.coli. harboring pEASY-E2-Control(inducted 5 h with IPTG inducement); 2: Total protein expressed by E.coli. harboring pEASY-E2-MsSQE1ΔN25 (without IPTG inducement); 3: Soluble protein expressed by E.coli. harboring pEASY-E2- MsSQE1ΔN25 (inducted 5 h with IPTG inducement); 4: Inclusion membrane protein expressed by E.coli. harboring pEASY-E2- MsSQE1ΔN25 (inducted 5 h with IPTG inducement); 5: Total protein expressed by E.coli. harboring pEASY-E2- MsSQE1(without IPTG inducement); 6: Soluble protein expressed by E.coli. harboring pEASY-E2-MsSQE1(inducted 5 h with IPTG inducement); 7: Inclusion membrane protein expressed by E.coli. harboring pEASY-E2-MsSQE1 (inducted 5 h with IPTG inducement)); Arrows represent expression of MsSQE1 protein