生物炭连续施用对农田土壤氮转化微生物及N<sub>2</sub>O排放的影响

生物炭连续施用对农田土壤氮转化微生物及N₂O排放的影响

董成,陈智勇,谢迎新,张阳阳,缑培欣,杨家蘅,马冬云,王晨阳,郭天财

Effects of Successive Biochar Addition to Soil on Nitrogen Functional Microorganisms and Nitrous Oxide Emission

DONG Cheng,CHEN ZhiYong,XIE YingXin,ZHANG YangYang,GOU PeiXin,YANG JiaHeng,MA DongYun,WANG ChenYang,GUO TianCai

表1 荧光定量PCR引物及扩增条件

Table 1 Primers and thermal profiles used in this study

扩增基因 Target	引物 Primer	序列 (5′— 3′) Sequence (5′- 3′)	荧光定量PCR程序 Real-time PCR profiles	参考文献 References
AOA	Arch-amoA F	STAATGGTCTGGCTTAGACG	95℃ 5 min;95℃ 30 s,58℃	[31]
AOA	Arch-amoA R	GCGGCCATCCATCTGTATGT	30 s,72℃ 1 min,35 cycles	[31]
AOB	amoA-1F	GGGGTTTCTACTGGTGGT	95℃ 5 min;95℃ 30 s,58℃	[32]
AOB	amoA-2R	CCCCTCKGSAAAGCCTTCTTC	30 s,72℃ 1 min,35 cycles	[32]
nirK	nirK1F	GGMATGGTKCCSTGGCA	95℃ 5 min;95℃ 30 s,58℃	[33]
nirK	nirK5R	GCCTCGATCAGRTTRTGG	30 s,72℃ 1 min,35 cycles	[33]
nirS	Cd3aF	GTSAACGTSAAGGARACSGG	95℃ 5 min;95℃ 30 s,58℃	[34]
nirS	R3cdR	GASTTCGGRTGSGTCTTGA	30 s,72℃ 1 min,35 cycles	[34]
nosZ	nosZ-F	AGAACGACCAGCTGATCGACA	95℃ 5 min;95℃ 30 s,60℃	[35]
nosZ	nosZ-R	TCCATGGTGACGCCGTGGTTG	30 s,72℃ 1 min,35 cycles	[35]