稻纵卷叶螟颗粒体病毒的持续感染及检测
韩光杰(),刘琴,李传明,祁建杭,徐彬,陆玉荣,徐健()
The Persistent Infection and Detection of Cnaphalocrocis medinalis Granulovirus in Cnaphalocrocis medinalis
HAN GuangJie(),LIU Qin,LI ChuanMing,QI JianHang,XU Bin,LU YuRong,XU Jian()

图3. 不同方式处理昆虫体表病毒检测
1—4:CnmeGV处理的稻纵卷叶螟蛹The pupa of C. medinalis treated with CnmeGV;5—8:稻纵卷叶螟健康蛹The healthy pupa of C. medinalis;9—12:10%甲醛处理稻纵卷叶螟蛹(病毒处理)10 min The infected pupa treated with 10% formaldehyde for 10 min;13—16:10%甲醛处理蛹表30 min The infected pupa treated with 10% formaldehyde for 30 min;17—20:经碱处理并使用DNase处理蛹表30 min The infected pupa treated with Lye-DNase for 30 min;21—24:10%甲醛处理蛹表16 h The infected pupa treated with 10% formaldehyde for 16 h

Fig. 3. Detection of CnmeGV on the surface of pupa treated with different ways