紫花苜蓿MsNST的克隆及对木质素与纤维素合成的功能分析
蒋旭(
),崔会婷,王珍,张铁军,龙瑞才,杨青川,康俊梅()
),崔会婷,王珍,张铁军,龙瑞才,杨青川,康俊梅()
Cloning and Function Analysis of MsNST in Lignin and Cellulose Biosynthesis Pathway from Alfalfa
JIANG Xu(),CUI HuiTing,WANG Zhen,ZHANG TieJun,LONG RuiCai,YANG QingChuan,KANG JunMei()
),CUI HuiTing,WANG Zhen,ZHANG TieJun,LONG RuiCai,YANG QingChuan,KANG JunMei()
图8. 过表达MsNST对细胞壁厚度及木质素、纤维素和总糖含量的影响
A：间苯三酚-盐酸对细胞壁中木质素染色;B：Image J软件对细胞壁厚度统计;C：拟南芥茎木质素含量;D：拟南芥茎纤维素含量测定;E：拟南芥茎总糖含量测定,n=10,使用t检验做显著性分析“*”代表P<0.05,“**”代表P<0.01;bar为±SD
Fig. 8. Effects of overexpression MsNST on cell wall thickness, lignin, cellulose and total sugar content in transgenic Arabidopsis thaliana
A: Stem cell walls of Arabidopsis stain by phloroglucinol-hydrochloric acid; B: Statistical analysis of interfascicular fiber cell wall thickness; C: Determination of lignin content of transgenic Arabidopsis stem; D: Determination of cellulose content in transgenic Arabidopsis stem; E: Determination of total sugar content in transgenic Arabidopsis stem
