紫花苜蓿MsNST的克隆及对木质素与纤维素合成的功能分析
蒋旭(),崔会婷,王珍,张铁军,龙瑞才,杨青川,康俊梅()
Cloning and Function Analysis of MsNST in Lignin and Cellulose Biosynthesis Pathway from Alfalfa
JIANG Xu(),CUI HuiTing,WANG Zhen,ZHANG TieJun,LONG RuiCai,YANG QingChuan,KANG JunMei()

图7. 转基因拟南芥鉴定与表型分析
A:5周龄野生型与转基因拟南芥植株,bar=2cm;B:转基因拟南芥阳性鉴定;C:不同株系转基因拟南芥相对表达量分析;D:过表达MsNST基因抑制拟南芥下胚轴生长,n=30,误差为±SD下同,“*” 和“**”分别代表t检验P<0.05和<0.01。下同;E:7周野生型拟南芥与转基因拟南芥株高,鲜重统计分析

Fig. 7. Identification and phenotypic analysis of transgenic Arabidopsis thaliana
A: Phenotype of five-week old transgenic Arabidopsis in green house,bars=2cm; B: Ampification of MsNST gene from transgenic Arabidopsis; C: The expression level of MsNST in transgenic Arabidopsis; D: Over expression MsNST result in growth inhibition of Arabidopsis hypocotyls. n=30,error bars replace ± standard deviation. “*” or “**” on behalf of P value < 0.05 or <0.01 by stutent t test analysis; E: Statistics analysis of plant height and fresh weight of transgenic Arabidopsis thaliana and WT