人溶菌酶密码子优化及其在牛乳腺细胞中高效表达

田媛,王力,龙凤,昝林森,成功

Codon Optimization of Human Lysozyme and High-Efficiency Expression in Bovine Mammary Cells

TIAN Yuan,WANG Li,LONG Feng,ZAN LinSen,CHENG Gong

表3 牛乳蛋白及人溶菌酶基因密码子组成分析

Table 3 Codon composition analysis of bovine milk proteins and human lysozyme gene

Bos taurus A3s T3s C3s G3s GC3s GC CAI ENc αs1酪蛋白alpha-s1 casein 0.311 0.461 0.290 0.258 0.408 0.445 0.227 52.300 αs2酪蛋白alpha-s2 casein 0.253 0.453 0.340 0.335 0.484 0.417 0.317 49.970 β酪蛋白beta casein 0.201 0.421 0.342 0.298 0.505 0.512 0.234 48.750 κ-酪蛋白 kappa casein 0.399 0.412 0.243 0.194 0.341 0.430 0.240 58.250 乳球蛋白lactoglogbulin 0.079 0.147 0.574 0.519 0.825 0.586 0.349 36.270 α乳清白蛋白alpha-lactalbumin 0.283 0.349 0.395 0.337 0.526 0.441 0.281 55.510 乳铁蛋白lactotransferrin 0.170 0.242 0.410 0.449 0.671 0.561 0.262 47.650 Mean ± SEM 0.242±0.039 0.355±0.045 0.371±0.040 0.341±0.042 0.537±0.062 0.485±0.026 0.273±0.017 49.814±2.668 人溶菌酶 human lysozyme 0.3143 0.4032 0.2742 0.2323 0.407 0.473 0.193 50.270